healthy female hipsc lines Search Results


kute-4 female skin fibroblast-derived human ipsc (hipsc) line  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures kute-4 female skin fibroblast-derived human ipsc (hipsc) line
Kute 4 Female Skin Fibroblast Derived Human Ipsc (Hipsc) Line, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/healthy+female+hipsc+lines/kute+4+female+skin+fibroblast+derived+human+ipsc++hipsc++line/pmc07611574-164-2-13
Average 90 stars, based on 1 article reviews
kute-4 female skin fibroblast-derived human ipsc (hipsc) line - by Bioz Stars, 2026-10
90/100 stars
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healthy female hipsc lines  (BioResource International Inc)
90
BioResource International Inc healthy female hipsc lines
( a ) Schematic of scalable expansion of XCI-rescued lines. Day 0 represents the day of transfection. XCI-rescued lines were cultured to reach over 10⁷ cells in the presence of RI and assessed for XCI status by IF of H3K27me3 and OCT4. ( b ) Quantification of XCI-positive cells. At least 584 cells were analyzed per line to confirm XCI restoration. ( c ) XCI status assessment in 10 <t>RIKEN</t> <t>BRC</t> quality-controlled (QC) female iPSC lines. The right panel shows quantification of XCI status by IF for H3K27me3, restricted to OCT4-positive cells. At least 382 cells were analyzed per line. ( d ) XCI rescue in HPS3810 and HPS4115 lines. The representative image of H3K27me3 foci with OCT4 staining (Left lower panel). The right panel shows the percentage of cells with H3K27me3 foci within the OCT4-positive population before sub-cloning. The blue dotted line represents the starting population shown in (c). Scale bar: 20 μm. ( e ) Model of dual inhibition approach for XIST reactivation. Transient inhibition of TP53 and DNMT1 during the NHEJ process boosts genome editing efficiency and prevents DNA methylation maintenance, thereby enhancing the efficiency of XIST reactivation.
Healthy Female Hipsc Lines, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/healthy+female+hipsc+lines/healthy+female+hipsc+lines/bio_rxiv__2024__11__04__622001-71-8-9
Average 90 stars, based on 1 article reviews
healthy female hipsc lines - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


( a ) Schematic of scalable expansion of XCI-rescued lines. Day 0 represents the day of transfection. XCI-rescued lines were cultured to reach over 10⁷ cells in the presence of RI and assessed for XCI status by IF of H3K27me3 and OCT4. ( b ) Quantification of XCI-positive cells. At least 584 cells were analyzed per line to confirm XCI restoration. ( c ) XCI status assessment in 10 RIKEN BRC quality-controlled (QC) female iPSC lines. The right panel shows quantification of XCI status by IF for H3K27me3, restricted to OCT4-positive cells. At least 382 cells were analyzed per line. ( d ) XCI rescue in HPS3810 and HPS4115 lines. The representative image of H3K27me3 foci with OCT4 staining (Left lower panel). The right panel shows the percentage of cells with H3K27me3 foci within the OCT4-positive population before sub-cloning. The blue dotted line represents the starting population shown in (c). Scale bar: 20 μm. ( e ) Model of dual inhibition approach for XIST reactivation. Transient inhibition of TP53 and DNMT1 during the NHEJ process boosts genome editing efficiency and prevents DNA methylation maintenance, thereby enhancing the efficiency of XIST reactivation.

Journal: bioRxiv

Article Title: Highly efficient XIST reactivation in female hPSC by transient dual inhibition of TP53 and DNA methylation during Cas9 mediated genome editing

doi: 10.1101/2024.11.04.622001

Figure Lengend Snippet: ( a ) Schematic of scalable expansion of XCI-rescued lines. Day 0 represents the day of transfection. XCI-rescued lines were cultured to reach over 10⁷ cells in the presence of RI and assessed for XCI status by IF of H3K27me3 and OCT4. ( b ) Quantification of XCI-positive cells. At least 584 cells were analyzed per line to confirm XCI restoration. ( c ) XCI status assessment in 10 RIKEN BRC quality-controlled (QC) female iPSC lines. The right panel shows quantification of XCI status by IF for H3K27me3, restricted to OCT4-positive cells. At least 382 cells were analyzed per line. ( d ) XCI rescue in HPS3810 and HPS4115 lines. The representative image of H3K27me3 foci with OCT4 staining (Left lower panel). The right panel shows the percentage of cells with H3K27me3 foci within the OCT4-positive population before sub-cloning. The blue dotted line represents the starting population shown in (c). Scale bar: 20 μm. ( e ) Model of dual inhibition approach for XIST reactivation. Transient inhibition of TP53 and DNMT1 during the NHEJ process boosts genome editing efficiency and prevents DNA methylation maintenance, thereby enhancing the efficiency of XIST reactivation.

Article Snippet: We used healthy female hiPSC lines from the RIKEN BioResource Center (BRC), which were quality-controlled (QC) and available for global distribution.

Techniques: Transfection, Cell Culture, Staining, Subcloning, Inhibition, DNA Methylation Assay